Where


The Exposure and Biological Analysis Facility (EBAF) is located on the fourth floor of the Children's Research Institute on the campus of the Medical College of Wisconsin and Children's Hospital of Wisconsin and at UWM's Great Lakes WATER Institute, 600 E. Greenfield Avenue, Milwaukee.

If you are interested in learning more, please contact the facility director, Dr. Paula North at (414) 266-2255 or Dr. Henry Tomasiewicz at (414) 382-1700. 

 

PALM_microsc_rs2

Zeiss P.A.L.M. Microbeam III Laser Capture Microdissection System (with 10x Fluar, 20x Neofluar, 40x Plan Neofluar and 63x Plan Neofluar objectives and with DAPI, TRITC and FITC filters.)

LSM-Non-Meta_rs

Zeiss LSM510 laser scanning confocal inverted microscrope equipped with 405 diode (for DAPI), multiline argon (458nm, 477nm, 488nm, 514 m), DPSS561 (568nm), and red HeNe (633nm) lasers, and EC Plan Neofluar 5x and 10x, Plan Apo 20x, LD D-Apochromat 40x and C-Apo 63x objectives.

Axiovert-200M_rs

Zeiss Axiovert 200M inverted microscope with Axiocam MrC camera (with 2.5x Plan Neofluar, 10x Plan APO, 20x LD-A Plan, 20x Plan Apo, and 40x Plan Apo objectives and filter sets for DAPi, rhodamine, and FITC). Fees for the Axiovert 200 are currently subsidized to users by the core.

 

Exposure and Biological Analysis Facility Core

Core Director: Paula North, MD PhD

The Exposure and Biological Analysis Facility Core provides Center investigators with professional-quality histological and immunohistochemical staining, a variety of state-of-the-art wide-field and laser-scanning microscopic imaging systems, and specimen storage support services that facilitate their individual and collaborative basic and translational investigations in a timely and cost effective manner. These services provide investigators with the diagnostic and analysis tools needed to further their research around environmental health concerns.

The Core provides:

  • A fertile environment of group, one-on-one, and web-based interactive opportunities that will catalyze new interactions between basic and clinical scientists and thus promote the development of new translational research projects.
  • Enriched pre-college education in the community through direct and web-based educational outreach activities by EHSC Center participants, facilitated by sharing of appropriate high quality photos provided by this facility Core that depict the effects of environmental toxicants on embryonic development.
  • Provide a human tissue and blood specimen storage mechanism using the resources of a preexisting, pathology-directed Pediatric BioBank and Analytical Tissue Core to support the Integrative Health Sciences Facility Core.

Imaging services provide Center investigators with:

  • Convenient assisted and unassisted access to state-of-the-art widefield, confocal, and multi-photon laser scanning microscope systems.
  • Convenient assisted and unassisted access to a well-maintained fluorescence and brightfield-equipped laser capture microdissection (LCM) system.
  • Management and maintenance of all the shared microscope systems and training and assistance for the EHSC Core Users and their staff in the effective and safe use of these systems.

Histology services provide Center investigators with:

  • A wide spectrum of timely, consistent, and high-quality histology services including, but not limited to, grossing, processing, embedding, cutting, and frozen sectioning.
  • Education of personnel in EHSC Core User laboratories in the proper collection, fixation, orientation, and transport of tissue specimens for histological processing and imaging studies.
  • Expertise to assist investigators in interpreting histological and immunohistochemical findings in tissue sections from humans and animal models of human environmentally-modulated disease.

If you are interested in learning more, please contact Dr. Paula North at (414) 266-2255 or pnorth@mcw.edu.

LSM 510 Meta_rs360

Zeiss LSM510 META NLO multiphoton laser scanning microscope equipped with an Argon mulit-line laser 9458nm, 477nm, 514nm), a DPSS561 (568nm) laser, a red HeNe laser (633nm), and a Coherent Chameleon Ultra II Ti: sapphire laser, rapidly tunable between 680 and 1080 nm. This system is outfitted with a Zeiss XL-3 incubator to assure stable environmental control during long incubations and with Zeiss EC Plan Neofluar 5x and 10x, Plan Apo 20x, C-Apo 40x and W Plan-Apo objectives. Software systems include Axiovision 4.6 and the basic LSM software, supplemented by modules for multichannel florescence, extended focus, interactive measurement, deconvolution, multi-time, and rapid 3D reconstruction.