Electron Microscope Laboratory

Electron Microscopes
Hitachi H-600 Transmission Electron Microscope (TEM)
  • operable in conventional, scanning transmission electron microscopy (STEM), and scanning electron microscopy (SEM) modes
  • accelerating voltage to 100 kV in 25 kV increments
  • capable of digital image acquisition (1024 x 1024 maximum resolution) in STEM and SEM modes
  • specimen holders: standard, dual, rotational (goniometer), low background (beryllium) current metering, and cryotransfer (see below)
Hitachi S-4800 Ultra High Resolution Cold Cathode Field Emission Scanning Electron Microscope (FE-SEM)
  • operable in secondary electron (SE), backscattered electron (BE), and STEM modes
  • capable of simultaneous brightfield and darkfield STEM imaging
  • digital image acquisition
  • exceeds manufacturer’s guaranteed resolutions of 1 nm at 15 kV, 2 nm at 1 kV, and 1.4 nm at 1 kV with beam deceleration on site (standard gold on carbon test specimen)
Energy-dispersive X-ray Spectrometry (EDS)
Hitachi S-4800 FE-SEM is equipped with a Bruker Quantax EDS system
  • thin window silicon drift detector (SDD) allows the detection of elements Boron and higher
  • point analysis, line scan analysis and digital dot mapping capabilities
TEM Specimen Preparation
  • RMC MT 7000 Ultramicrotome (with cryoultramicrotomy attachment - see below)
  • RMC MT 7 Ultramicrotome
  • Reichert Ultracut E Ultramicrotome
  • glass knife makers (LKB, RMC)
  • LKB Multiplate
SEM Specimen Preparation
  • Critical point dryers (Sorvall, Balzers, Tousimis)
  • Sputter coaters (SPI, CS Mini Coater, EMITECH K575x with carbon coating attachment)
  • Vacuum evaporator (Edwards)
Cryo Specimen Preparation
  • RMC Propane Jet Freezer
  • Leica AFS Freeze Substitution Apparatus
  • RMC CR-21 attachment for RMC MT 7000 ultramicrotome
  • Oxford CT 3500 Cryo Transfer System for Hitachi H-600 TEM
Light Microscopes
  • Leica TCS SP2 Confocal System installed on a Leica upright compound microscope. Microscope equipped for DIC, phase contrast and epifluorescence microscopy.
    • 3 lasers provide 6 excitation lines for confocal imaging (458 nm, 476 nm, 488 nm, 514 nm, 543 nm and 633 nm)
    • 3 PMT channels for confocal acquisition, one transmitted light PMT
    • Spot RT color digital camera for image capture in other modes
  • Olympus BH-2 upright compound microscope equipped for epifluorescence microscopy with a 100 watt mercury burner and 3 filter cubes (Olympus B L0910, B L0911 and B L012)
  • Olympus IMT - 2 inverted compound microscope equipped with phase contrast optics.
  • Olympus PM 10AD photomicrographic system for acquisition of 35 mm photographs with Olympus BH-2 and Olympus IMT-2 microscopes.
  • Zeiss Large Universal upright microscope. Equipped for epifluorescence microscopy and 35 mm photography (Zeiss MC 63 camera system).
Image Analysis, Storage and Output
  • Fully equipped B&W darkroom (including condenser enlarger, point-source enlarger)
  • Windows based image analysis computer and software (Photoshop, Optimas, Metamorph)
  • Epson Perfection V750 Pro scanner
  • Lexmark 1200 dpi laser printer
  • Hewlett Packard color inkjet printer
  • Epson Stylus Photo R800 color printer
  • Polaroid 8000 Digital Palette film recorder
  • Wet lab for TEM and SEM specimen preparation including incubators, ovens, balances, stereo and light microscopes, centrifuges, microcentrifuge, pH meter, hot plates, stir plates, fume hoods, etc.