Electron Microscope Laboratory
Hitachi H-600 Transmission Electron Microscope (TEM)
Hitachi S-4800 Ultra High Resolution Cold Cathode Field Emission Scanning Electron Microscope (FE-SEM)
Energy-dispersive X-ray Spectrometry (EDS)
- operable in conventional, scanning transmission electron microscopy (STEM), and scanning electron microscopy (SEM) modes
- accelerating voltage to 100 kV in 25 kV increments
- capable of digital image acquisition (1024 x 1024 maximum resolution) in STEM and SEM modes
- specimen holders: standard, dual, rotational (goniometer), low background (beryllium) current metering, and cryotransfer (see below)
- operable in secondary electron (SE), backscattered electron (BE), and STEM modes
- capable of simultaneous brightfield and darkfield STEM imaging
- digital image acquisition
- exceeds manufacturer’s guaranteed resolutions of 1 nm at 15 kV, 2 nm at 1 kV, and 1.4 nm at 1 kV with beam deceleration on site (standard gold on carbon test specimen)
Hitachi S-4800 FE-SEM is equipped with a Bruker Quantax EDS system
TEM Specimen Preparation
- thin window silicon drift detector (SDD) allows the detection of elements Boron and higher
- point analysis, line scan analysis and digital dot mapping capabilities
- RMC MT 7000 Ultramicrotome (with cryoultramicrotomy attachment - see below)
- RMC MT 7 Ultramicrotome
- Reichert Ultracut E Ultramicrotome
- glass knife makers (LKB, RMC)
- LKB Multiplate
- Critical point dryers (Sorvall, Balzers, Tousimis)
- Sputter coaters (SPI, CS Mini Coater, EMITECH K575x with carbon coating attachment)
- Vacuum evaporator (Edwards)
- RMC Propane Jet Freezer
- Leica AFS Freeze Substitution Apparatus
- RMC CR-21 attachment for RMC MT 7000 ultramicrotome
- Oxford CT 3500 Cryo Transfer System for Hitachi H-600 TEM
- Leica TCS SP2 Confocal System installed on a Leica upright compound microscope. Microscope equipped for DIC, phase contrast and epifluorescence microscopy.
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- 3 lasers provide 6 excitation lines for confocal imaging (458 nm, 476 nm, 488 nm, 514 nm, 543 nm and 633 nm)
- 3 PMT channels for confocal acquisition, one transmitted light PMT
- Spot RT color digital camera for image capture in other modes
- Olympus BH-2 upright compound microscope equipped for epifluorescence microscopy with a 100 watt mercury burner and 3 filter cubes (Olympus B L0910, B L0911 and B L012)
- Olympus IMT - 2 inverted compound microscope equipped with phase contrast optics.
- Olympus PM 10AD photomicrographic system for acquisition of 35 mm photographs with Olympus BH-2 and Olympus IMT-2 microscopes.
- Zeiss Large Universal upright microscope. Equipped for epifluorescence microscopy and 35 mm photography (Zeiss MC 63 camera system).
- Fully equipped B&W darkroom (including condenser enlarger, point-source enlarger)
- Windows based image analysis computer and software (Photoshop, Optimas, Metamorph)
- Epson Perfection V750 Pro scanner
- Lexmark 1200 dpi laser printer
- Hewlett Packard color inkjet printer
- Epson Stylus Photo R800 color printer
- Polaroid 8000 Digital Palette film recorder
- Wet lab for TEM and SEM specimen preparation including incubators, ovens, balances, stereo and light microscopes, centrifuges, microcentrifuge, pH meter, hot plates, stir plates, fume hoods, etc.