Post-doctoral Fellow/Associate, Whitehead Institute for Biomedical Research, 2004-2011
Ph.D., University of Wisconsin-Madison, Molecular and Environmental Toxicology, 2004
B.S., University of Massachusetts-Amherst, Biology, 1998
Office: Lapham N515
(A) (B) (C, D)
Our lab applies genetic, developmental, molecular, and cell biological approaches to study brain morphogenesis in zebrafish (Danio rerio) embryos. The challenge is to determine the mechanisms of brain morphogenesis within a live organism. Zebrafish biology provides a robust in vivo model for sophisticated and feasible developmental studies. Zebrafish have rapid development, short generation time, and transparent embryos allowing for in vivo live imaging.Specific Areas of Interest:
- Determining the mechanisms for how non-muscle myosin II regulates cell shortening during the formation of the midbrain-hindbrain boundary constriction.
- Discovery and characterization of the role for unconventional myosins in brain morphogenesis and disease.
- Analysis of the role of molecular motors in neurite outgrowth during brain development.
Gutzman, J.H. and Sive, H. Epithelial relaxation mediated by the myosin phosphatase regulator Mypt1 is required for brain ventricle lumen expansion and hindbrain morphogenesis. Development. 2010 Mar;137(5):795-804. **Featured Article.
Glazer AM, Wilkinson AW, Backer CB, Lapan SW, Gutzman J.H., Cheeseman IM, Reddien PW. The Zn finger protein Iguana impacts Hedgehog signaling by promoting ciliogenesis. Developmental Biology. 2010 Jan 1;337(1):148-56.
Gutzman, J.H. and Sive, H. Zebrafish brain ventricle injection. Journal of Visualized Experiments. 2009 Apr 6;(26).
Lowery, L.A., DeRienzo, G., Gutzman, J.H. and Sive, H. Characterization and classification of zebrafish brain morphology mutants. Anatomical Record. 2009 Jan; 292(1):94-106.
Gutzman, J.H.*, Graeden, E.G.*, Lowery, L.A., Holley, H.S. and Sive, H. Formation of the zebrafish midbrain-hindbrain boundary constriction requires laminin-dependent basal constriction. Mechanisms of Development. 2008 Nov-Dec;125(11-12):974-83. *These authors contributed equally to this work.