Mark J. McBride
Mark J. McBride
Professor
Microbiology/Molecular Biology

B.S., Univ. of Rochester 1980
Ph.D., Univ. of Wisconsin 1987
Postdoctoral Fellow Univ. of California-Berkeley 1991

Office: Lapham N307
Phone: 414-229-5844
FAX: 414-229-3926
Email: mcbride@uwm.edu
Vitae:

Research Interests

The molecular mechanism by which swimming bacteria are propelled through liquid media by rotating flagella is understood relatively well. Gliding motility (movement of cells over surfaces without the aid of flagella) is a trait common to many bacteria, yet the mechanisms responsible for gliding motility are poorly understood. My lab uses the techniques of genetics, molecular biology, biochemistry, and microscopy to determine the mechanism of Flavobacterium johnsoniae gliding motility. We developed techniques to allow genetic manipulation of this organism, and have used these techniques to isolate nonmotile mutants and to identify the genes that are altered in these mutants. These genes code for proteins that make up the gliding motility apparatus ('motor') that propels the cells. We use antibodies raised against these proteins to localize the components of the motor, determine how they interact, and visualize the gliding motility apparatus in cells. Based on our results we have developed a model for Flavobacterium gliding that involves the active movement of adhesive fibrils along the cell surface. We also identified a novel protein secretion system that is involved in assembly of the motility apparatus.

In addition to studies on gliding motility, we are also investigating other aspects of the biology of gliding bacteria. These bacteria are abundant in many environments and some have characteristics that make them important organisms to study. Examples of applied projects include studies of chitin digestion by F. johnsoniae, studies of cellulose digestion by Cytophaga hutchinsonii, and genetic analysis of the fish pathogens Flavobacterium psychrophilum and Flavobacterium columnare.

Selected Publications

A. Shrivastava, J. J. Johnston, J. M. van Baaren, and M. J. McBride. 2013. Flavobacterium johnsoniae GldK, GldL, GldM, and SprA are required for secretion of the cell-surface gliding motility adhesins SprB and RemA. J. Bacteriol. 195:3201-3212. http://jb.asm.org/content/195/14/3201.long

D. Nakane, K. Sato, H. Wada, M. J. McBride, and K. Nakayama. 2013. Helical flow of surface protein required for bacterial locomotion. Proc. Natl. Acad. Sci. USA. 110:11145-11150. http://www.pnas.org/content/110/27/11145.long

M. J. McBride, and Y. Zhu. 2013. Gliding motility and Por secretion system genes are widespread among members of the phylum Bacteroidetes. J. Bacteriol. 195:270-278. http://jb.asm.org/content/195/2/270.long

A. Shrivastava, R. G. Rhodes, S. Pochiraju, D. Nakane, and M. J. McBride. 2012.Flavobacterium johnsoniae RemA is a mobile cell-surface lectin involved in gliding. J. Bacteriol. 194:3678-3688. http://jb.asm.org/content/194/14/3678.long

R. G. Rhodes, M. N. Samarasam, E. J. van Groll, and M. J. McBride. 2011. Mutations in Flavobacterium johnsoniae sprE result in defects in gliding motility and protein secretion. J. Bacteriol. 193:5322-5327. http://jb.asm.org/content/193/19/5322.long

R. G. Rhodes, H. G. Pucker, and M. J. McBride. 2011. Development and use of a gene deletion strategy for Flavobacterium johnsoniae to identify the redundant gliding motility genes remF, remG, remH, and remI. J. Bacteriol. 193:2418-2428. http://jb.asm.org/content/193/10/2418.long

R. G. Rhodes, S. S. Nelson, S. Pochiraju, and M. J. McBride. 2011.Flavobacterium johnsoniae sprB is part of an operon spanning the additional gliding motility genes sprC, sprD, and sprF. J. Bacteriol. 193:599-610. http://jb.asm.org/content/193/3/599.long

R. G. Rhodes, M. N. Samarasam, A. Shrivastava, J. M. van Baaren, S. Pochiraju, S. Bollampalli, and M. J. McBride. 2010. Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB. J. Bacteriol. 192:1201-1211. http://jb.asm.org/content/192/5/1201.long

K. Sato, M. Naito, H. Yukitake, H. Hirakawa, M. Shoji, M. J. McBride, R. G. Rhodes, and K. Nakayama. 2010. A protein secretion system linked to bacteroidete gliding motility and pathogenesis. Proc. Natl. Acad. Sci. USA. 107:276-281. http://www.pnas.org/content/107/1/276.full

M. J. McBride, G. Xie, E. C. Martens, A. Lapidus, B. Henrissat, R. G. Rhodes, E. Goltsman, W. Wang, J. Xu, D. W. Hunnicutt, A. M. Staroscik, T. R. Hoover, Y. Q. Cheng, and J. L. Stein. 2009. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl. Environ. Microbiol. 75:6864-6875. http://aem.asm.org/content/75/21/6864.long

K. F. Jarrell and M. J. McBride. 2008. The surprisingly diverse ways that prokaryotes move. Nature Rev. Microbiol. 6:466-476. http://www.nature.com/nrmicro/journal/v6/n6/abs/nrmicro1900.html

S. S. Nelson, S. Bollampalli, and M. J. McBride. 2008. SprB is a cell-surface component of the Flavobacterium johnsoniae gliding motility machinery. J. Bacteriol. 190:2851-2857. http://jb.asm.org/content/190/8/2851.long

J. Liu, M. J. McBride, and S. Subramaniam. 2007. Cell-surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. J. Bacteriol. 189:7503-7506. http://jb.asm.org/content/189/20/7503.long

S. S. Nelson, P. P. Glocka, S. Agarwal, D. P. Grimm, and M. J. McBride. 2007.Flavobacterium johnsoniae SprA is a cell-surface protein involved in gliding motility.J. Bacteriol.189:7145-7150. http://jb.asm.org/content/189/19/7145.long

G. Xie, D. C. Bruce, J. F. Challacombe, O. Chertkov, J. C. Detter, P. Gilna, C. S. Han, S. Lucas, M. Misra, G. L. Myers, P. Richardson, R. Tapia, N. Thayer, L. S. Thompson, T. S. Brettin, B. Henrissat, D. B. Wilson, and M. J. McBride. 2007. Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii. Appl. Environ. Microbiol. 73: 3536-3546. http://aem.asm.org/content/73/11/3536.long