Mark J. McBride
Professor
Microbiology/Molecular Biology
Ph.D., Univ. of Wisconsin 1987
Postdoctoral Fellow Univ. of California-Berkeley 1991
Office: Lapham N307
Phone: 414-229-5844
FAX: 414-229-3926
Email: mcbride@uwm.edu
Vitae:
Research Interests
The molecular mechanism by which swimming bacteria are propelled through liquid media by rotating flagella is understood relatively well. Gliding motility (movement of cells over surfaces without the aid of flagella) is a trait common to many bacteria, yet the mechanisms responsible for gliding motility are poorly understood. My lab uses the techniques of genetics, molecular biology, biochemistry, and microscopy to determine the mechanism of Flavobacterium johnsoniae gliding motility. We developed techniques to allow genetic manipulation of this organism, and have used these techniques to isolate nonmotile mutants and to identify the genes that are altered in these mutants. These genes code for proteins that make up the gliding motility apparatus ('motor') that propels the cells. We use antibodies raised against these proteins to localize the components of the motor, determine how they interact, and visualize the gliding motility apparatus in cells. Based on our results we have developed a model for Flavobacterium gliding that involves the active movement of adhesive fibrils along the cell surface.In addition to studies on gliding motility, we are also investigating other aspects of the biology of gliding bacteria. These bacteria are abundant in many environments and some have characteristics that make them important organisms to study. Examples of applied projects include studies of chitin degradation by F. johnsoniae , studies of cellulose degradation by Cytophaga hutchinsoni, and genetic analysis of the fish pathogen Flavobacterium psychrophilum.
Selected Publications
R. G. Rhodes, M. N. Samarasam, A. Shrivastava, J. M. van Baaren, S. Pochiraju, S. Bollampalli, and M. J. McBride. 2010. Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB. J. Bacteriol. 192:1201-1211. http://jb.asm.org/cgi/content/abstract/192/5/1201
K. Sato, M. Naito, H. Yukitake, H. Hirakawa, M. Shoji, M. J. McBride, R. G. Rhodes, and K. Nakayama. 2010. A protein secretion system linked to bacteroidete gliding motility and pathogenesis. Proc. Natl. Acad. Sci. USA. 107:276-281. http://www.pnas.org/content/107/1/276.full
M. J. McBride, G. Xie, E. C. Martens, A. Lapidus, B. Henrissat, R. G. Rhodes, E. Goltsman, W. Wang, J. Xu, D. W. Hunnicutt, A. M. Staroscik, T. R. Hoover, Y. Q. Cheng, and J. L. Stein. 2009. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl. Environ. Microbiol. 75:6864-6875. http://aem.asm.org/cgi/content/abstract/75/21/6864
K. F. Jarrell and M. J. McBride. 2008. The surprisingly diverse ways that prokaryotes move. Nature Rev. Microbiol. 6:466-476. http://www.nature.com/nrmicro/journal/v6/n6/abs/nrmicro1900.html
S. S. Nelson, S. Bollampalli, and M. J. McBride. 2008. SprB is a cell-surface component of the Flavobacterium johnsoniae gliding motility machinery. J. Bacteriol. 190:2851-2857. http://jb.asm.org/cgi/content/abstract/190/8/2851
J. Liu, M. J. McBride, and S. Subramaniam. 2007. Cell-surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. J. Bacteriol. 189:7503-7506. http://jb.asm.org/cgi/content/short/189/20/7503
G. Xie, D. C. Bruce, J. F. Challacombe, O. Chertkov, J. C. Detter, P. Gilna, C. S. Han, S. Lucas, M. Misra, G. L. Myers, P. Richardson, R. Tapia, N. Thayer, L. S. Thompson, T. S. Brettin, B. Henrissat, D. B. Wilson, and M. J. McBride. 2007. Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii. Appl. Environ. Microbiol. 73: 3536-3546. http://aem.asm.org/cgi/content/abstract/73/11/3536
T. F. Braun, M. K. Khubbar, D. A. Saffarini and M. J. McBride. 2005. Flavobacterium johnsoniae gliding motility genes identified by mariner mutagenesis. J. Bacteriol. 187: 6943-6952. http://jb.asm.org/cgi/content/abstract/187/20/6943
T. F. Braun and M. J. McBride. 2005. Flavobacterium johnsoniae GldJ is a lipoprotein that is required for gliding motility. J. Bacteriol. 187:2628-2637. http://jb.asm.org/cgi/content/abstract/187/8/2628
M. J. McBride and T. F. Braun. 2004. GldI is a lipoprotein that is required for Flavobacterium johnsoniae gliding motility and chitin utilization. J. Bacteriol.186:2295-2302. Abstract
B. Alvarez, P. Secades, M. J. McBride, and J. A. Guijarro. 2004. Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum. Appl. Environ. Microbiol. 70:581-587. Abstract
K. Sato, M. Naito, H. Yukitake, H. Hirakawa, M. Shoji, M. J. McBride, R. G. Rhodes, and K. Nakayama. 2010. A protein secretion system linked to bacteroidete gliding motility and pathogenesis. Proc. Natl. Acad. Sci. USA. 107:276-281. http://www.pnas.org/content/107/1/276.full
M. J. McBride, G. Xie, E. C. Martens, A. Lapidus, B. Henrissat, R. G. Rhodes, E. Goltsman, W. Wang, J. Xu, D. W. Hunnicutt, A. M. Staroscik, T. R. Hoover, Y. Q. Cheng, and J. L. Stein. 2009. Novel features of the polysaccharide-digesting gliding bacterium Flavobacterium johnsoniae as revealed by genome sequence analysis. Appl. Environ. Microbiol. 75:6864-6875. http://aem.asm.org/cgi/content/abstract/75/21/6864
K. F. Jarrell and M. J. McBride. 2008. The surprisingly diverse ways that prokaryotes move. Nature Rev. Microbiol. 6:466-476. http://www.nature.com/nrmicro/journal/v6/n6/abs/nrmicro1900.html
S. S. Nelson, S. Bollampalli, and M. J. McBride. 2008. SprB is a cell-surface component of the Flavobacterium johnsoniae gliding motility machinery. J. Bacteriol. 190:2851-2857. http://jb.asm.org/cgi/content/abstract/190/8/2851
J. Liu, M. J. McBride, and S. Subramaniam. 2007. Cell-surface filaments of the gliding bacterium Flavobacterium johnsoniae revealed by cryo-electron tomography. J. Bacteriol. 189:7503-7506. http://jb.asm.org/cgi/content/short/189/20/7503
G. Xie, D. C. Bruce, J. F. Challacombe, O. Chertkov, J. C. Detter, P. Gilna, C. S. Han, S. Lucas, M. Misra, G. L. Myers, P. Richardson, R. Tapia, N. Thayer, L. S. Thompson, T. S. Brettin, B. Henrissat, D. B. Wilson, and M. J. McBride. 2007. Genome sequence of the cellulolytic gliding bacterium Cytophaga hutchinsonii. Appl. Environ. Microbiol. 73: 3536-3546. http://aem.asm.org/cgi/content/abstract/73/11/3536
T. F. Braun, M. K. Khubbar, D. A. Saffarini and M. J. McBride. 2005. Flavobacterium johnsoniae gliding motility genes identified by mariner mutagenesis. J. Bacteriol. 187: 6943-6952. http://jb.asm.org/cgi/content/abstract/187/20/6943
T. F. Braun and M. J. McBride. 2005. Flavobacterium johnsoniae GldJ is a lipoprotein that is required for gliding motility. J. Bacteriol. 187:2628-2637. http://jb.asm.org/cgi/content/abstract/187/8/2628
M. J. McBride and T. F. Braun. 2004. GldI is a lipoprotein that is required for Flavobacterium johnsoniae gliding motility and chitin utilization. J. Bacteriol.186:2295-2302. Abstract
B. Alvarez, P. Secades, M. J. McBride, and J. A. Guijarro. 2004. Development of genetic techniques for the psychrotrophic fish pathogen Flavobacterium psychrophilum. Appl. Environ. Microbiol. 70:581-587. Abstract