David N. Frick

David N. Frick

Professor

Office: Chemistry 333
Phone: 414-229-6670
e-mail: frickd@uwm.edu
Website: http://uwm.edu/~frickd

Degree(s):

Ph.D., The Johns Hopkins University

Research Description:

My lab mainly studies the biochemistry of viral proteins and small molecules that interact with them. Our main interest, presently, is in targeting a helicase encoded by the hepatitis C virus (HCV). Helicases are motor proteins that separate DNA and RNA duplexes and dislodge proteins bound to nucleic acids in reactions fueled by ATP hydrolysis. We also study other helicases from related viruses and human cells, and other viral proteins such as polymerases, proteases and capsid proteins. The goal of most projects in the lab is either to understand how these proteins help copy viral genomes or to understand how small molecule drugs block virus growth by directly interfering with these important enzymes.

Molecular model of a fluorescent NS3 helicase inhibitor
A molecular model of a fluorescent NS3 helicase inhibitor bound to the protein overlaid on a picture of human cells infected with HCV. The human cells also contain the helicase inhibitor, which is visualized in the cytoplasm using fluorescence microscopy.

Techniques used in our lab:

  • Recombinant DNA technology
  • Protein purification
  • Enzymology
  • Steady state and transient state kinetics
  • Tissue Culture
  • Fluorescence Microscopy
  • Quantitative RT-PCR
  • High throughput screening
  • Absorbance spectroscopy
  • Fluorescence spectroscopy
  • TR-FRET, alpha-screen and FP assays
  • Biocalorimetry
  • Molecular Modeling

For more information, see our Lab Website, or this UWM News article on our research.

Selected Publications:

Temorarily Unavailible.