Marius Schmidt


Professional Website


Phone: (414) 229-4338

Office: 454

Research Interests:
Biophysics; Macromolecular Crystallography; Time-resolved Macromolecular Crystallography; Spectroscopy

Related links:

Marius Schmidt


Professor Schmidt is using physical methods to investigate biological molecules. He is concentrating mainly on static and time-resolved macromolecular crystallography to investigate the structure, dynamics and kinetics of proteins. Schmidt is also developing new, sophisticated software for the analysis of the time-resolved (4-dimensional) X-ray data. Results from crystallography are corroborated by (time or energy resolved) spectroscopic methods.

Biographical Sketch:

Marius Schmidt obtained his Dipl. in Biology in 1990 at Johannes-Gutenberg-University, Mainz (Germany). He received his Dr. rer. Nat. in 1996 at Technische Universität München, Garching (Germany), where later he became habilitated in Experimental Physics in 2004. In 2008 he obtained an habilitation in the French system in Theoretical, physical and analytical Chemistry.

From 1996 to 1998 Schmidt worked as a scientist for the Physics Department at Technische Universität München (Germany) and from 1998 to 2001 as a research associate for the Department of Biochemistry and Molecular Biology at the University of Chicago. From 2001 to 2007 he worked as a scientist and Privatdozent for the Physics Department at Technische Universität München (TUM), (Germany). 

Schmidt has been at the University of Wisconsin-Milwaukee since 2007, where he holds the rank of Professor.

Selected Publications:


  • M. Schmidt and D. K. Saldin
    "Enzyme transient state kinetics in crystal and solution from the perspective of a time-resolved crystallographer"
    Structural Dynamics 1, 024701 (2014)
  • Y. O. Jung, J. H. Lee, J. Kim, M. Schmidt, K. Moffat, V. Srajer and H. Ihee
    "Volume-conserving trans-cis isomerization pathways in photoactive yellow protein visualized by picosecond X-ray crystallography"
    Nature Chemistry 5, 212-220 (2013)
  • M. Schmidt, V. Srajer, R. Henning, H. Ihee, N. Purwar, J. Tenboer and S. Tripathi
    "Protein enegy landscapes determined by five-dimensional crystallography"
    Acta Cryst. D 69, 2534-2542 (2013)
  • Y. Youngblut, E. T. Judd, V. Srajer, B. Sayyed, T. Goelzer, S. J. Elliot, M. Schmidt and A. Pachero
    "Laue Structure of S. Oneidensis ccNIR"
    Journal of Biological Inorganic Chemistry, 17, 647-672 (2012)
  • S. Tripathi, V. Srajer, R. Henning, N. Purwar and M. Schmidt
    "The pH dependence of the PYP photocycle - a time-resolved crystallographic investigation"
    Biophysical Journal 102(2), 325-332 (2012)
  • N. Purwar, J. McGarry, A. Pacheco and M. Schmidt
    "The Interaction of Nitric Oxide with Catalase: Structural and Kinetic Analysis"
    Biochemistry 50, 4491-4503 (2011)
  • M. Schmidt, T. Graber, R. Henning, and V. Srajer
    "Five-Dimensional Crystallography"
    Acta Cryst. A 66, 198-206 (2010)
  • M. Schmidt,A. Patel, Y. Zhao and W. Reuter
    "Structural basis for the photochemistry of alfa-phycoerythrocyanin"
    Biochemistry, 46, 416-423 (2007)
  • M. Schmidt, K. Nienhaus, R. Pahl, A. Krasselt, U. Nienhaus, F. Parak, and V. Srajer
    "Ligand migration pathway and protein dynamics in myoglobin: A time-resolved crystallographic study on L29W MbCO"
    Proc. Natl. Acad. Sci. USA 13, 11704-11709 (2005)
  • M. Schmidt, R. Pahl, S. Anderson, V. Srajer, Z. Ren and K. Moffat
    "Protein Kinetics: Structures of Intermediates and Reaction Mechanism by Time-Resolved X-ray Crystallography"
    Proc. Natl. Acad. Sci. USA 101, 4799-4804 (2004)
  • M. Schmidt, S. Rajagopal, Z. Ren and K. Moffat
    "Application of Singular Value Decomposition to the Analysis of Time-Resolved Macromolecular X-Ray Data"
    Biophysical Journal 84, 2112-2129 (2003)
Books Chapters
  • M. Schmidt
    "Structure Based Enzyme Kinetics by Time-Resolved X-ray crystallography"
    In: Ultrashort Laser Pulses in Medicine and Biology (eds: W. Zinth, M. Braun, P. Gilch). Springer Verlag, 201-241 (2008)
  • M. Schmidt, R. Pahl, H. Ihee, and V. Srajer
    "Protein ligand interaction probed by time-resolved X-ray structure determination"
    In: Methods in Molecular Biology, Vo. 305, protein-ligand interactions: Methods and Applications (G.U. Nienhaus ed.), Humana Press, Totowa, NJ, 115-154 (2005).