Office of Undergraduate Research

Polygalacturonate-modulated Regulators for T3SS Gene Expression

Objectives: 1.

Identify the polygalacturonate-modulated regulators for T3SS gene expression.

2. Investigate the gene-expression pattern between two different genes in one bacterial strain using a dual-labeling-FACS assay.

Tasks and responsibilities:

Student tasks:Identify the components that connect the pectinolytic and T3SS regulatory pathways (Objectives 1 and 2). Responsibility:Molecular cloning, gene expression assays, record data and write lab reports.