Insights into the localization and activation of transcription factor HAC1
The unfolded protein response (UPR) is a cellular stress response. The UPR is induced in response to an
accumulation of unfolded proteins in the lumen of endoplasmic reticulum (ER) and the induction of yeast UPRinvolves activation of ER resident protein IRE1. The active yeast IRE1 cleaves Hac1 mRNA to remove an
inhibitory intron and relieves the translational block. HAC1p is a transcription factor that translocates and binds tothe UPRE (unfolded protein response element) located upstream of a number of ER-stress responsive genes toactivate transcription. The specific goals of this research project are: (1) to gain the molecular insights into thelocalization of HAC1p and (2) to identify binding partners of HAC1p.