Steady-state analysis of nitrite reduction by the enzyme ccNiR
We are interested in the enzymes that some bacteria use to catalyze ammonia-nitrite interconversion, an ecologically important process. In the bacterium S. oneidensis, Nitrite reduction to ammonia is carried out by the enzyme cytochrome c nitrite reductase" (ccNiR), which we can produce in large quantities and high purity. Flors main project for the summer will be to study nitrite reduction at the ccNiR active site using steady-state methods, and a variety of electron donors. In the past nitrite reduction in vitro has been performed using the powerful reductant methyl viologen monocation radical. We want to see how the reaction rate varies when weaker reductants are used.
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