Small molecule inhibitors of type III secretion in Erwinia amylovora
Objective: Evaluate the inhibitory effects and determine the regulatory mechanisms of the newly synthesized compounds against T3SS.
To date, along with 43 commercially available phenolic compounds, 58 compounds have been synthesized based on the structure-activity relationships of T3SS inhibitors previously identified in our lab. Among these 101 compounds, several compounds showed either an inducing or inhibitory effect against T3SS of three plant pathogens including Erwinia amylovora. Using E. amylovora as a model microorganism, we will study the modes of action of T3SS inducers/inhibitors on the T3SS components and regulators.
Methodology: Molecular cloning, flow cytometry, and Northern blot will be used to measure the expression of genes encoding T3SS components and regulators.