Office of Undergraduate Research

Transgenic zebrafish as a Tool for Looking at the Effects of Environmental Toxicants on Development

We use gene trapping to create transgenic zebrafish expressing fluorescent proteins in different cells or tissues during developments. The light emitted by the fluorescent protein allows one to follow its expression, and the cells expressing the fluorescent protein during development using a fluorescent microscope. We can determine the effects of environmental toxicants on particular cell types expressing the fluorescent proteins via fluorescent microscopy. Also, we use PCR based techniques to locate where in the zebrafish genome the transgene is inserted.

This project is located at the School of Freshwater Science: 600 E. Greenfield Ave. Milwaukee, WI 53204

***Campus Shuttle Service to The School of Freshwater Science is now available!***

https://www4.uwm.edu/freshwater/

Tasks and responsibilities:

Zebrafish husbandry: Including feeding, spawning, collecting and observing embryos and larval fish. Microscopy: Using stereomicroscopes, inverted and upright microscopes and recording observations using high end CCD cameras. Molecular Biology: RNA/DNA isolation, polymerase chain reaction Bioinformatics.